Bernstein Center Freiburg

PhD Seminar

March 17, 2020
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Bernstein Seminar
ESTHER FLORES ►

IMTEK | Albert-Ludwigs University Freiburg

Combining Microelectrode Arrays, Patch clamp and Calcium Imaging for understanding the integration of the synaptic input in cultured networks

Tuesday, March 17, 2020

17:15h



Hosted by Katharina Heining

vCal iCal

Bernstein Center Freiburg
Lecture Hall (ground floor)
Hansastr. 9a
79104 Freiburg


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Abstract

Neuronal cell cultures are used in order to model basic functions and mechanisms of the brain. In this framework, neuronal cells are extracted from wistar rat (at postnatal day 1) primary cortex, and seeded on microelectrode arrays (MEAs). These cells self-organize in forming functioning networks, showing properties and electrophysiological activity similar to those in vivo. The MEAs are capable of both recording electrophysiological activity, and stimulating these networks with small electrical pulses. We can modify the neurites and neurons spatial distribution on the MEAs. For this purpose we modify the protein kinase C (PKC) activity, with the PKC agonist phorbol-12-myristate- 13-acetate (PMA) and with the PKC inhibitor Gödecke6976.Since changing the spatial distribution of neurons and neurites has influence in the connectivity of the network and thus the synaptic input into individual neurons we can analyze synaptic dynamics with whole cell patch clamp recordings during the network activities on the MEAs.PKC activity is modulated by intracellular calcium dynamics. We can asses the dynamics of the citoplasmatic calcium with the genetically encoded calcium indicators GCaMP6s.