The Bernstein Center for Computational Neuroscience Freiburg

Announcement for the next
Informal Seminar
Dr. Fabian Müller
Pharos GmbH, Ulm

The Effect of Sirolimus on the Expression of Intercellular Adhesion Molecule-1 in Human Coronary Blood Vessel Wall Cells

Friday, November 7th, 2008
10:30 h TIME
Epilepsiezentrum im Neurozentrum
Universitätsklinik Freiburg
Background: Sirolimus (SRL) has been used successfully to inhibit restenosis in drug eluting stents. The current study reports on the effects of SRL on the expression of intercellular adhesion molecule-1 (ICAM-1) after stimulation by tumor necrosis factor-a (TNF-a).

Methods: Human umbilical vein endothelial cells (HUVEC), human coronary artery endothelial cells (HCAEC) and human coronary artery media smooth muscle cells (HCMSMC) were seeded into 6-well dishes (5 x 104 /cm2 cells). SRL (0.01, 0.1, 1, 10, 100, 1000 ng/mL) was added to the cultures for a period of 18 h. During the last 6 h of SRL incubation, the expression of ICAM-1 was stimulated by adding TNF-a (20 ng/mL). For detection of ICAM-1, a fluoresceine isothiocyanate conjugated antibody against ICAM-1 was used. 104 cells per well were analysed for expression of ICAM-1 in flow cytometry.

Results: Expression of ICAM-1 was dose dependently and significantly inhibited in HUVEC, HCAEC and HCMSMC.

Conclusion: Reduction of restenosis rates after implantation of SRL-eluting stents could partly be caused by inhibition of ICAM-1 expression.
The talk is open to the public. Guests are cordially invited!